Journal: Poultry Science

Article Title: New insights into ovarian regression-related mitochondrial dysfunction in the late-laying period

doi: 10.1016/j.psj.2025.104938

Figure Lengend Snippet: Differences in the regulation of ferroptosis under ovarian regression. (A) GSH and (B) Fe 2+ levels in the ovary ( n = 6). (C) Representative western blots of GPX4, SLC7A11, and COX2. (D) GPX4/β-actin. (E) SLC7A11/β-actin. (F) COX2/β-actin. (G) Representative blots of CAT, SOD1, and SOD2. (H) CAT/β-actin. (I) SOD1/β-actin. (J) SOD2/β-actin ( n = 3). * P < 0.05.

Article Snippet: The proteins were boiled, separated by SDS-PAGE, transferred onto a polyvinylidene fluoride membrane and incubated with primary antibodies SIRT1 (1:1,000, 13161-1-AP, Proteintech, Chicago, IL, USA), SIRT3 (1:500, SIR3-301AP, Thermo, Waltham, MA, USA), TFAM (1:2,000, A3173, ABclonal, Wuhan, China), PGC-1α (1:500, AF5395, Affinity, MN, USA), GPX4 (1:2,000; 67763-1, Proteintech), SLC7A11 (1:1,000, A13685, ABclonal), CAT (1:500; orb334965, Biorbyt, Cambridge, UK), COX2 (1:500; bs-10411R, Boston, MA, USA), SOD1 (1:500; AF5198, Affinity), SOD2 (1:500; bs-23402R, Bioss).

Techniques: Western Blot